armcx3  (Proteintech)

Journal: Heliyon

Article Title: ARMCX3 regulates ROS signaling, affects neural differentiation and inflammatory microenvironment in dental pulp stem cells

doi: 10.1016/j.heliyon.2024.e37079

Figure Lengend Snippet: Primers information.

Article Snippet: ARMCX3 , 25705-1-AP , Proteintech , USA , 1:1000.

Techniques:

Journal: Heliyon

Article Title: ARMCX3 regulates ROS signaling, affects neural differentiation and inflammatory microenvironment in dental pulp stem cells

doi: 10.1016/j.heliyon.2024.e37079

Figure Lengend Snippet: Antibodies information.

Article Snippet: ARMCX3 , 25705-1-AP , Proteintech , USA , 1:1000.

Techniques:

Journal: Heliyon

Article Title: ARMCX3 regulates ROS signaling, affects neural differentiation and inflammatory microenvironment in dental pulp stem cells

doi: 10.1016/j.heliyon.2024.e37079

Figure Lengend Snippet: ARMCX3 knockdown facilitates neural differentiation of hDPSCs. (A) The expression level of ARMCX3 in Pg-LPS-treated hDPSCs tested via qRT-PCR assay. (B–E) The expression level of ARMCX3 tested by qRT-PCR and Western blot assay . (F) The detection of neural differentiation ability of Pg-LPS-treated hDPSCs. (G) The expression level of neural-specific marker GFAP (neural-specific marker) tested via IF assay. (H) The expression level of GFAP and βIII-Tubulin (neuron specific marker) tested via qRT-PCR assay. Scale bar = 50 μm * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: ARMCX3 , 25705-1-AP , Proteintech , USA , 1:1000.

Techniques: Knockdown, Expressing, Quantitative RT-PCR, Western Blot, Marker

Journal: Heliyon

Article Title: ARMCX3 regulates ROS signaling, affects neural differentiation and inflammatory microenvironment in dental pulp stem cells

doi: 10.1016/j.heliyon.2024.e37079

Figure Lengend Snippet: ARMCX3 knockdown reduces inflammation response in hDPSCs. (A) The protein level of pro-inflammatory cytokines in Pg-LPS-treated hDPSCs determined by ELISA assay. (B) The mRNA level of COX2 and iNOS in Pg-LPS-treated hDPSCs tested via qRT-PCR assay. ** p < 0.01, *** p < 0.001.

Article Snippet: ARMCX3 , 25705-1-AP , Proteintech , USA , 1:1000.

Techniques: Knockdown, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

Journal: Heliyon

Article Title: ARMCX3 regulates ROS signaling, affects neural differentiation and inflammatory microenvironment in dental pulp stem cells

doi: 10.1016/j.heliyon.2024.e37079

Figure Lengend Snippet: ARMCX3 knockdown suppresses ROS signal in Pg-LPS-treated hDPSCs. (A) The production level of ROS in Pg-LPS-treated hDPSCs detected by IF assay. (B&C) The content of oxidative stress related indicators in Pg-LPS-treated hDPSCs detected by specific kits. (D) The level of Ca 2+ in Pg-LPS-treated hDPSCs. * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: ARMCX3 , 25705-1-AP , Proteintech , USA , 1:1000.

Techniques: Knockdown

Journal: Heliyon

Article Title: ARMCX3 regulates ROS signaling, affects neural differentiation and inflammatory microenvironment in dental pulp stem cells

doi: 10.1016/j.heliyon.2024.e37079

Figure Lengend Snippet: Inhibition of ROS blocks the effects of ARMCX3 on neural differentiation of hDPSCs. (A&B) The expression level of ARMCX3 tested by qRT-PCR and Western blot assay . (C) The production level of ROS detected by IF assay. (D) The detection of neural differentiation ability of hDPSCs. (E) The expression level of GFAP (neural-specific marker) tested via IF assay. (F) The expression level of GFAP and βIII-Tubulin (neuron specific marker) tested via qRT-PCR assay. Scale bar = 50 μm * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: ARMCX3 , 25705-1-AP , Proteintech , USA , 1:1000.

Techniques: Inhibition, Expressing, Quantitative RT-PCR, Western Blot, Marker

Journal: Heliyon

Article Title: ARMCX3 regulates ROS signaling, affects neural differentiation and inflammatory microenvironment in dental pulp stem cells

doi: 10.1016/j.heliyon.2024.e37079

Figure Lengend Snippet: Inhibition of ROS blocks the effects of ARMCX3 on inflammation microenvironment in hDPSCs. (A) The expression level of pro-inflammatory cytokines in Pg-LPS-treated hDPSCs determined by ELISA assay. (B) The mRNA level of COX2 and iNOS in Pg-LPS-treated hDPSCs tested via qRT-PCR assay. ** p < 0.01, *** p < 0.001.

Article Snippet: ARMCX3 , 25705-1-AP , Proteintech , USA , 1:1000.

Techniques: Inhibition, Expressing, Enzyme-linked Immunosorbent Assay, Quantitative RT-PCR

Journal: Cancers

Article Title: ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity

doi: 10.3390/cancers13051110

Figure Lengend Snippet: ARMCX3 deletion reduces the susceptibility of mice to high-fat diet (HFD)-induced obesity and metabolic alterations. ( A ) Armcx3 mRNA levels in livers of mice exposed to regular feeding conditions (Control), overnight fasting (Fast), high-fat diet (HFD) or 24 h after intraperitoneal injection with 2 mg/kg tunicamycin (Tun) (left). Immunoblot of ARMCX3 protein levels and loading control (Ponceau staining) in livers from mice fed HFD or treated with tunicamycin for 24 h (right) (For uncropped immunoblots here and thereafter, see File S1). ( B ) ARMCX3 mRNA levels in liver from healthy individuals (control, n = 19) and NASH patients ( n = 24). ( C ) Schematic representation of the study design: 6-week-old f Armcx3/Cre- (Control) and ARMCX3-KO mice (tamoxifen-induced Cre activity) (KO) were fed low-fat diet (LFD) or HFD for 16 weeks, (left). Body weight curves (left, central), body weight gain (right, central) and dietary energy intake (right). ( D ) Glycemia (left), insulinemia (middle) and HOMA-IR index (right). ( E ) Glucose tolerance curves. ( F ) Representative microscopic pictures of H&E-stained livers (left), and quantification of the percentage of total area occupied by fat (right). Magnification: 10×. ( G ) Serum ALT levels. Bars indicate means ± SEM; n = 6–8 mice per group. T-student, Mann-Whitney or Two-Way ANOVA test with Tukey or Dunnet post hoc corrections were used. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 and **** p ≤ 0.0001 for comparisons between diets or treatments, and # p ≤ 0.05, ### p ≤ 0.001 for comparisons between genotypes.

Article Snippet: Samples were immunobloted with rabbit anti-ARMCX3 antibody 1/1000 (25705-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Control, Injection, Western Blot, Staining, Activity Assay, MANN-WHITNEY

Journal: Cancers

Article Title: ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity

doi: 10.3390/cancers13051110

Figure Lengend Snippet: ARMCX3 deletion decreases diethylnitrosamine (DEN)-induced tumorigenesis and steatohepatitis in mice exposed to HFD. Data were obtained from 7-month-old f Armcx3/Cre- (Control) or ARMCX3-KO mice (KO) treated with DEN and maintained on LFD or HFD. ( A ) Schematic representation of the study design: 15-day-old f Armcx3/Cre- (Control) and ARMCX3-KO mice were injected with 25 mg/kg DEN and, beginning at week 6, fed LFD or HFD for 24 weeks. ( B ) Body weight curves (left) and body weight gain (right). ( C ) Representative pictures of livers (top) and quantifications of tumor number, size and maximal tumor size (bottom). Representative images and lipid accumulation quantification in liver sections stained with hematoxylin-eosin ( D ) and Oil-Red O ( E ). Scale bar: 100 µm. ( F ) Serum ALT levels. Bars show means ± SEM; n = 9–13 mice per group. Two-Way ANOVA test with Tukey post hoc correction was used. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 and **** p ≤ 0.0001 for comparisons between diets or treatments.

Article Snippet: Samples were immunobloted with rabbit anti-ARMCX3 antibody 1/1000 (25705-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Control, Injection, Staining

Journal: Cancers

Article Title: ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity

doi: 10.3390/cancers13051110

Figure Lengend Snippet: ARMCX3 deletion increases apoptosis and macrophage infiltration in hepatic tumors from HFD-fed mice. Data correspond to liver sections from 7-month-old f A3/Cre- (Control) or ARMCX3-KO mice treated with DEN and maintained on LFD or HFD. Representative liver sections from non-tumor (NT) and tumor (T) sections (left) and quantifications (right) of immunostaining for Ki67 ( A ), BrdU ( B ), cleaved caspase-3 ( C ) and F4/80 ( D ). Data are means ± SEM of 2–8 mice. Two-Way ANOVA test with Tukey post hoc correction was used. * p ≤ 0.05 and ** p ≤ 0.01. Magnification: 13×.

Article Snippet: Samples were immunobloted with rabbit anti-ARMCX3 antibody 1/1000 (25705-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Control, Immunostaining

Journal: Cancers

Article Title: ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity

doi: 10.3390/cancers13051110

Figure Lengend Snippet: ARMCX3 deletion affects ERK signaling in hepatic tumors from HFD-fed mice. ( A ) Representative staining of β-catenin in liver sections from Control or ARMCX3-KO mice maintained on HFD. Sections include both non-tumor (NT) and tumor (T) regions and magnifications are shown. Scale bars: 100 µm and 50 µm (magnified). ( B ) Immunoblot analysis of the phosphorylation of ERK and p38 (representing pathway activation) in control and ARMCX3-KO mice exposed to HFD. Shown are representative immunoblot images (left) and quantification of phosphorylated/total ratios for ERK and p38 (right). Bars indicate means ± SEM; n = 2–4 mice per group. Two-Way ANOVA test with Tukey post hoc correction was used. * p ≤ 0.05 for comparisons between Control and KO samples.

Article Snippet: Samples were immunobloted with rabbit anti-ARMCX3 antibody 1/1000 (25705-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Staining, Control, Western Blot, Phospho-proteomics, Activation Assay

Journal: Cancers

Article Title: ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity

doi: 10.3390/cancers13051110

Figure Lengend Snippet: ARMCX3 deletion reduces the viability, clonality and invasivity of HCC cells in vitro. ( A ) ARMCX3 mRNA levels in Hep3B and SNU423 HCC cells 24 h and 48 h after transfection with ARMCX3 siRNA versus negative control siRNA (top dotted line), **** p ≤ 0.0001. ( B ) Colony-forming assays performed with Hep3B and SNU423 cells transfected with negative control siRNA (Control) or ARMCX3 siRNA. ( C ) Representative images of wound-healing assay results obtained using control and ARMCX3-KD Hep3B cells. Magnification: 10× ( D ) Percentage viability of control and ARMCX3-KD Hep3B and SNU423 cells at 24 h, 48 h and 72 h after transfection. Bars show the means ± SEM of three independent cell culture points. Two-Way ANOVA test with Dunnett post hoc correction was used. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 and **** p ≤ 0.0001.

Article Snippet: Samples were immunobloted with rabbit anti-ARMCX3 antibody 1/1000 (25705-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: In Vitro, Transfection, Negative Control, Control, Wound Healing Assay, Cell Culture

Journal: Cancers

Article Title: ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity

doi: 10.3390/cancers13051110

Figure Lengend Snippet: ARMCX3 expression correlates with the proliferative capacity in hepatocytes. ( A ) MTT and colony-forming assays in SNU423 cells overexpressing ARMCX3 at 48 h post-transduction. ( B ) PCNA protein levels in the cells described in ( A ), presented as representative immunoblots (top) and quantification (bottom) of PCNA and ARMCX3 levels. ( C ) PCNA protein levels in primary hepatocytes overexpressing ARMCX3 at 48 h post-transduction, presented as representative immunoblots (left) and quantification (right) of PCNA and ARMCX3 levels. ( D ) PCNA and ARMCX3 protein levels in livers of mice subjected to partial hepatectomy at 24 h, 48 h or 72 h post-surgery, and in livers of mice treated with CCl 4 for 48 h; representative immunoblots (top) and quantifications (bottom) are presented. ( E ) Pearson’s correlation between ARMCX3 and PCNA protein levels in hepatocytes ( n = 30). ( F ) ARMCX3 mRNA levels in HepG2 cells treated with palmitic acid (0.3 mM) or oleic acid (0.6 mM) for 24 h. Ponceau staining, which was used as a loading control in the immunoblots, is shown in . T-student or Mann-Whitney test with Dunnett post hoc corrections were used. * p ≤ 0.05, ** p ≤ 0.01, *** p ≤ 0.001 and **** p ≤ 0.0001.

Article Snippet: Samples were immunobloted with rabbit anti-ARMCX3 antibody 1/1000 (25705-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Expressing, Transduction, Western Blot, Staining, Control, MANN-WHITNEY

Journal: Cancers

Article Title: ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity

doi: 10.3390/cancers13051110

Figure Lengend Snippet: ARMCX3-induced hepatocyte proliferation involves SOX9. ( A ) Immunodetection of ARMCX3 and SOX9 in SOX9 pull-down samples from HepG2 cells transduced with Ad-GFP or Ad-ARMCX3 at 48 h post-transduction. ( B ) ARMCX3, SOX9 and PCNA levels in HepG2 cells at 48 h after cells were transduced with Ad-GFP or Ad-ARMCX3 and transfected with scrambled siRNA (Control) or SOX9 siRNA. Representative immunoblots (top) and quantification (bottom) are shown. Bars indicate means ± SEM of three independent experiments. Two-Way ANOVA test with Tukey post hoc correction was used. * p ≤ 0.05 for comparisons between siControl and siSOX9, and ## p ≤ 0.01 for comparisons between GFP and ARMCX3. ( C ) SOX9 levels in 7-month-old fArmcx3/Cre- (Control) or ARMCX3-KO mice (KO) treated with DEN and maintained on LFD or HFD for 24 weeks. Bars indicate means ± SEM; n = 9–13 mice per group. Two-Way ANOVA test with Tukey post hoc correction was used. ## p ≤ 0.01 for comparisons between genotypes.

Article Snippet: Samples were immunobloted with rabbit anti-ARMCX3 antibody 1/1000 (25705-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: Immunodetection, Transduction, Transfection, Control, Western Blot

Journal: Cancers

Article Title: ARMCX3 Mediates Susceptibility to Hepatic Tumorigenesis Promoted by Dietary Lipotoxicity

doi: 10.3390/cancers13051110

Figure Lengend Snippet: Schematic representation of the role of hepatic ARMCX3, in interaction with SOX9, in eliciting proliferative signals and hepatocarcinogenesis in response to lipotoxicity and hepatic damaging signals.

Article Snippet: Samples were immunobloted with rabbit anti-ARMCX3 antibody 1/1000 (25705-1-AP; Proteintech, Rosemont, IL, USA).

Techniques: